Video 4.

Monomeric EB3-NL-mVenus labels MT growing ends in vitro. In vitro MT plus-end tracking assays were performed with 1μM EB3-NL-mVenus and 15 μM tubulin. TIRFM images were acquired in a streaming mode with a 0.1-s exposure; the intensity of three consecutive frames was averaged.

Mammalian end binding proteins control persistent microtubule growth

Yulia Komarova, Christian O. De Groot, Ilya Grigoriev, Susana Montenegro Gouveia, E. Laura Munteanu, Joseph M. Schober, Srinivas Honnappa, Rubén M. Buey, Casper C. Hoogenraad, Marileen Dogterom, Gary G. Borisy, Michel O. Steinmetz, and Anna Akhmanova

DOI: 10.1083/jcb.200807179