CENP-C RNAi. This video shows S2 cells expressing H2B-GFP and mCherry-tubulin undergoing mitosis after CENP-C depletion. This video shows cells displaying chromosome misalignment, severe spindle formation defects, and failure of sister chromatid separation. Cytokinesis can proceed without proper chromosome segregation, resulting in a “cut” phenotype and severe aneuploidy. Stills are shown in Fig. 2. Bar, 15 µm.
