CID RNAi. This videos shows S2 cells expressing H2B-GFP and mCherry-tubulin undergoing mitosis after CID depletion. CID depletion results in chromosomes that are unable to attach to the spindle or move, and the chromosomes remain stalled in metaphase. The spindle elongates prematurely, and mitosis does not complete. Stills are shown in Fig. 2. Bar, 15 µm.