Control for RNAi experiments. This video shows that untreated S2 cells expressing H2B-GFP and mCherry-tubulin divide normally. The chromosomes condense and align at the metaphase plate, and sister chromatids are pulled toward opposite poles in anaphase as the spindle elongates. Chromosome segregation is completed timely and accurately. Stills are shown in Fig. 2. Bar, 15 µm.