GFP-RCA1 and mCherry-tubulin. This video shows the localization of GFP-CYCA during mitosis in S2 cells. GFP-RCA1 levels are high and diffuse within the nucleus before mitosis. Upon nuclear envelope breakdown, the GFP-RCA1 signal becomes more diluted, and it disappears in anaphase and finally reappears during cytokinesis when the nuclear envelope is reformed. Bar, 15 µm.
