GFP-CYCA and mCherry-tubulin. This video shows the localization of GFP-CYCA (expressed under the control of the endogenous CYCA promoter) during mitosis in S2 cells. GFP-CYCA localizes diffusely in the nucleus and accumulates at centromeres in prometaphase. After nuclear envelope breakdown, the diffuse GFP-CYCA signal decreases, and the centromeric staining becomes more visible. The GFP-CYCA signal decreases dramatically in anaphase, which is presumably caused by APC-dependent proteolysis. Bar, 15 µm.
