GFP-CAL1 and mCherry-tubulin. This video shows a mitotic S2 cell expressing GFP-CAL1 and mCherry-tubulin. GFP-CAL1 localizes at centromeres throughout mitosis. The fluorescence intensity of GFP-CAL1 appears to decrease during metaphase and increase again in telophase. Stills are shown in Fig. 1 B. Bar, 15 µm.
