Video 3.

FRAP of the PtdIns4P reporter GFP-Osh2^PH. This video, corresponding to still images in Fig. 2 B, demonstrates the ability of the PtdIns4P reporter GFP-2xPH^Osh2 (carried in a BY4741 background) to recover fluorescence after photobleaching. The demarcated area represents the portion of the cell where FRAP was measured. Frames were collected every 5 s and each frame is displayed every 0.1 s.

Assembly of the PtdIns 4-kinase Stt4 complex at the plasma membrane requires Ypp1 and Efr3

Dan Baird , Chris Stefan , Anjon Audhya , Sabine Weys , and Scott D. Emr

DOI: 10.1083/jcb.200804003