Three-dimensional reconstruction of z stacks from cells expressing GFP-Stt4. The mean number of GFP-Stt4–based PIK patches per cell is shown. Eight optical sections acquired at 4-µm intervals (3-s exposures) were reconstructed using the Volume View program (DeltaVision). The video illustrates several dense patches of GFP-Stt4 fluorescence at cortical sites of DBY42 yeast.
