Video 5.

Recombinant CENP-E–GFP is localized to the kinetochore in Xenopus extract spindles. Red, X-rhodamine–labeled tubulin; green, CENP-E–GFP. Video playback is 90× real time. A selected frame from this video is shown in Fig. 3 D.

CENP-E combines a slow, processive motor and a flexible coiled coil to produce an essential motile kinetochore tether

Yumi Kim , John E. Heuser , Clare M. Waterman , and Don W. Cleveland

DOI: 10.1083/jcb.200802189