FRAP analysis suggests post-Golgi membrane accumulations in the cleavage furrow consist of individual vesicles. BSC1 cells expressing VSVG-YFP were imaged by SDCM acquiring one confocal stack per 10 s (from Fig. 3 F). After 50 s, the accumulation of VSVG-labeled post-Golgi vesicles in the cleavage furrow was photobleached at 100% laser power for 200 bleach cycles. Playback is at eight frames per second.
