Transition of filopodia into the lamella visualized in a CAR fibroblast transfected with mCherry-actin and EGFP-fascin. Video corresponds to Fig. 2 (A–C). Arrowheads indicate two bundles that form from filopodia folding in opposite directions into the cell edge. As the cell edge advances, fascin dissociates from the bundles, which subsequently develop into stress fibers in the lamella cytoskeleton. Note also the bending and kinking of other filopodia in the lower half of the frame and the radial withdrawal of a long filopodium into the lamella. Time-lapse images were acquired every 10 s and the total duration of the video is 32 min. The display rate is 6 frames/s. Bar, 10 µm.
