HeLa cells expressing YFP-GPI were imaged in Ca2+-free DME. Cells were imaged with a spinning disk confocal microscope. 300 µg/ml Cherry-SLO was locally added with a micropipette to slowly release the toxin for ∼1 s while imaging. Confocal stacks with 10 optical sections of YFP-GPI (green) and Cherry-SLO (red) were acquired simultaneously for 2 min at 1 frame/3.7 s. (A) YFP-GPI. (B) Cherry-SLO. (C) Merge. Videos are displayed at 7 frames/s.
