NRK cell stably expressing hNup93-EGFP3 and transiently expressing IBB-DiHcRed imaged from metaphase to telophase. Images were taken approximately every 30 s. Outlines show the regions used for quantification that were obtained by thresholding on Hoechst-stained chromatin. The frame rate is 10 frames/s. See Fig. S2 for selected stills. Bar, 5 µm.
