Inhibition of p38 activity prevents formation of nascent keratin granules and disassembly of keratin granules in cells producing mutant keratins. Time-lapse recording of HK14R125C-EYFP fluorescence at left (inverse presentation) and corresponding phase contrast images together with overlaid EYFP-K14R125C fluorescence micrographs at right of MT5K14-26 cells before, during and after a short treatment with the p38 inhibitor SB202190 (100 µM; corresponding Fig. 10 B-B"). Images were recorded every 20 s and are displayed at 30 frames/s. Note the transient cessation of keratin granule formation and stabilization of pre-existing keratin granules upon drug administration.
