FRAP analysis reveals slow F-actin flux rates in actin tails in cof1-22 sla2Δ double mutant cells. (A) FRAP analysis of GFP-actin-labeled actin comet tails in two sla2Δ cells. Arrows indicate regions proximal to the cell cortex that were photobleached after 5 s. Interval between frames is 1 s. Total acquisition time was 20 s. (B) FRAP analysis of GFP-actin-labeled actin comet tails in two sla2Δ cof1-22 cells. Arrows indicate regions proximal to the cell cortex that were photobleached after 10 s. Interval between frames is 1 s. Total acquisition time was 60 s. Bars, 4 µm.
