Time-lapse video of semipermeabilized GFP-LAP2β HeLa cells incubated in mitotic CSF-arrested Xenopus egg extract supplemented with a 155-kD TRITC-labeled dextran in the presence of recombinant RCC1. Scans were taken every 5 min for 45 min (five frames/second). Corresponds to Fig. 4 A (right).
