CENP-A-SNAP loading initiates in telophase. Time-lapse video of the experiment shown in Fig. 3 E. Images were acquired at a 10-min intervals, as described in Materials and methods. Cell shown was imaged for 4 h 30 min. Replay speed is 3,600x (1 live hour per second). Top left to bottom right is YFP-CENP-C, TMR-Star labeled CENP-A-SNAP, merge, DIC (differential interference contrast).