Video 2.

MIM/IMD-induced filopodia are highly dynamic. Human osteosarcoma cell (U2OS) transfected with GFP-tagged MIM/IMD-L. GFP total internal reflection fluorescence is in red, and GFP epifluorescence in green. Speed is 15 frames/s. Frame is captured every 20 s.

Missing-in-metastasis and IRSp53 deform PI(4,5)P2-rich membranes by an inverse BAR domain–like mechanism

Pieta K. Mattila , Anette Pykäläinen , Juha Saarikangas , Ville O. Paavilainen , Helena Vihinen , Eija Jokitalo , and Pekka Lappalainen

DOI: 10.1083/jcb.200609176