360° rotation of a 3D reconstructed HeLa cell presented in Fig. 1 C. VacA leaves EEs and enters LEs within 120 min as described in Fig. 1. The left top rotation presents the merge between VacA (green) and EEA1 (blue). The middle top rotation presents the VacA indirect fluorescence in black and white. The right top rotation shows the EEA1 indirect fluorescence in black and white. The left bottom rotation presents the merge between VacA (green) and LAMP1 (blue). The middle bottom rotation presents the VacA indirect fluorescence in black and white. The right bottom rotation shows the LAMP1 indirect fluorescence in black and white. Each frame represents a clockwise rotation of 10° (36 total frames). The rotation is presented at six frames per second. Bars, 5 µm.