Video 1.

GAKIN transports PIP₃ liposomes via PIP₃BP in vitro. Time-lapse visualization of rhodamine-labeled PIP₃ liposomes (red) moving along microtubules (green) in the presence of GST-PIP₃BP and GAKIN motor-FHA (aa 1-557) recombinant proteins. Frames were collected every 1.0 s. The frames/s display rate was 10, and 30 frames were used for each video. Bar, 5 µm.

Transport of PIP3 by GAKIN, a kinesin-3 family protein, regulates neuronal cell polarity

Kaori Horiguchi , Toshihiko Hanada , Yasuhisa Fukui , and Athar H. Chishti

DOI: 10.1083/jcb.200604031