BDNF induces SV dispersal and increased splitting of SV clusters from stably localized synaptophysin-GFP-labeled puncta. Time-lapse confocal imaging of synaptophysin-GFP fluorescence over a 20-min period. Cells were treated with 100 ng/ml BDNF at time point 0 (circle around punctum). Diffusion begins at ∼3-4 min, and the time point when diffusion is maximal is indicated by a line along the major axis of the punctum. Arrows point to budding events, and large, white asterisks indicate newly stabilized synaptophysin-GFP puncta (stable over at least 5 min). Small, yellow asterisks indicate mobile SV clusters being transported along the axon. 1 frame is equivalent to 1 min.
