A time-course video showing histone-GFP-expressing embryos after the localized depletion of GW function by antibody injection at the anterior pole during interphase of NC10. Beginning at the point of injection, the majority of chromosomes fail to separate during mitosis and, in several cases, resolve into abnormal ringlike chromatin structures. With subsequent mitotic cycles, additional nuclei progressively further from the site of injection show the same failure. These abnormal mitotic figures then progressively break down. In addition, the remaining nuclei are anchored longer at the embryo cortex as first posterior and then anterior nuclei begin to move freely within the embryo cytoplasm and ultimately begin to fuse. Images were captured at 1 frame/10 s and are displayed at 20 frames/s (∼200x speed).
