Animated 3D video of Fig. 3. Video of the cell viewed at two different angles. Note the appearance of small protrusions in regions adjacent to the TGN and structures in the subapical domain that contain only GPI-GL-YFP (as evidenced by the red instead of yellow color). During the chase, there are two distinct populations of GPI-GL-YFP on the apical membrane: one is evenly distributed on the membrane, whereas the other is restricted in specific regions (likely representing newly arrived GPI-GL-GFP; Hannan, L.A., M.P. Lisanti, E. Rodriguez-Boulan, and M. Edidin. 1993. J. Cell Biol. 120:353-358).
