PAGFP-Ub-expressing cells with activation of an area in the nucleus. (see also Fig. 5 A). The indicated region was activated with the FRAP module of the Leica software by exposing the indicated region to the 405-nm laser. The following FRAP protocol was used: two prebleach scans at 488 nm, two activation scans at 405 nm, and 20 postbleach scans with 10-s intervals, followed by >20 postbleach scans every 45 s at 488-nm excitation.
