Enlarged area selected from Video 1 illustrates the three phases of DP-GFP assembly into the A431 cell-cell border. Images were captured every 1.5 min and the movie is shown at 6 frames/s. In phase I, fluorescence appears at new contact sites (green arrows). In phase II, particles appear in the cytoplasm after cell-cell contact (red arrows). In phase III, cytoplasmic particles incorporate into the maturing cell border (yellow arrows). Video corresponds with still images in Fig. 2 A.
