Hemocyte chemotaxis requires actin polymerization. Direct comparison of hemocyte motility after application of a control bead (left) and a Cytochalasin D-soaked bead (right). (left) Hemocyte recruitment to a control Heparin bead (red) implanted in a pxnGAL4 UAS-GFP embryo. Hemocytes (green) are highly motile, continually throwing out dynamic lamellipodia as they surround the bead. (right) Affect on hemocyte motility in a pxnGAL4 UAS-GFP embryo after implantation of a heparin bead (red) soaked in Cytochalasin D. In contrast to the control situation, no hemocytes are in contact with the bead, and the cells appear far less motile than their wild-type counterparts, moving at a slower rate and forming fewer and smaller membrane protrusions. Frames are captured every 3 min. Total elapsed time is 45 min. Stills from this movie are shown in Fig. 6.
