Fluorescence time-lapse video microscopy showing kinetochore movements in a spindle undergoing anaphase in an X. laevis egg extract depleted of histone H1. Note that kinetochore segregation and movement appear very similar to the control (Video S1). Kinetochores were marked by the addition of a fluorescently labeled antibody that recognizes centromeric histone CENP-A and appear as white dots. Frames were collected every 30 s and are displayed at 10 frames/s. Fixed samples of a similar experiment are shown in Fig. 5 C.
