Effect of repetitive UV flashes on [Ca2+]i and trajectory of 30 µM nimodipine-treated sperm loaded with caged cGMP. Movie is shown in real time (43.5 frames/s) apart from the images at which the UV flashes are applied, when a 2-s (0.5 frames/s) pause has been imposed. Five UV flashes applied in total. Color bar as in Fig. 1, rescaled to show fluorescence intensity levels between 3 and 17.
