Corresponds to images in Fig. 6 B. Spindle formation in a dynein-depleted cell with nonseparated centrosomes. Under this condition, the spindle initially forms as a fan-shaped structure, with MTs converging on the centrosome(s) only on one side of the chromosomes. However, within just a few minutes formation of prominent K-fibers is seen on the other side of the chromosomes. These K-fibers do not converge on a common point. Over time, the centrosomes detach from the spindle and then the K-fibers on the side of the spindle that was initially well focused becomes barrel shaped.
