Video 3.

Corresponds to images presented in Fig. 2. This cell initially contains a mono-oriented chromosome, which is positioned near the top spindle pole. A kinetochore fiber forms in association with the unattached kinetochore that faces away from centrosome-generated astral MTs. This fiber initially grows from the kinetochore toward the cell's periphery, but it then suddenly turns toward the bottom spindle pole and begins to glide poleward. As the result of the gliding, the fiber becomes incorporated into the spindle, and the chromosome congresses onto the metaphase plate.


Kinetochore-driven formation of kinetochore fibers contributes to spindle assembly during animal mitosis

Helder Maiato , Conly L. Rieder , and Alexey Khodjakov

DOI: 10.1083/jcb.200407090
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