Chromosome segregation occurs without anaphase A or significant poleward flux. Time-lapse images of an embryo expressing ß-tubulin::GFP in which a short region of anterior spindle microtubules were photobleached at anaphase onset. In this movie, posterior movement of the spindle was eliminated: the spindle in each frame of time-lapse images was rotationally aligned and recentered on the photobleached region. The photobleached region on spindle microtubules remains at a constant distance from the spindle pole, as it follows the spindle pole. Frames were acquired at 7-s intervals.
