Video 2.

Secretory granule motion observed by evanescent wave fluorescence microscopy. A PC-12 cell was cotransfected by NPY-GFP and Rab27A-Q78L and imaged 3 d later by EW-FM. False color is used. Images were collected at 2 frames/s for 60 s, and the sequence is accelerated 5 times on the video (10 frames/s).

Rab27A and its effector MyRIP link secretory granules to F-actin and control their motion towards release sites

Claire Desnos , Jean-Sébastien Schonn , Sébastien Huet , Viet Samuel Tran , Aziz El-Amraoui , Graça Raposo , Isabelle Fanget , Catherine Chapuis , Gaël Ménasché , Geneviève de Saint Basile , Christine Petit , Sophie Cribier , Jean-Pierre Henry , and François Darchen

DOI: 10.1083/jcb.200302157