Video 5.

Kymograph tracking of barbed ends during simulated polymerization of a branched actin filament network. Actin subunits within the network of filaments are shown as red arrowheads, barbed ends are shown as yellow arrowheads. As the branching network polymerizes over time (blue axis), the position of the barbed ends is traced in yellow, producing a branched kymograph similar to that produced during assembly and turnover of podosomes at the macrophage leading edge.


Macrophage podosomes assemble at the leading lamella by growth and fragmentation

James G. Evans , Ivan Correia , Olga Krasavina , Nicki Watson , and Paul Matsudaira

DOI: 10.1083/jcb.200212037
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