A, Dextranwave ona single optical sectionof a 4Ddataset(see Fig. 6).A starfish oocyte injectedwith Alexa 488 500-kDdextranwasimagedduring maturationinthree dimensionsover time.A selectedoptical sectionfrom the datasetisshown.Stackswere acquiredevery 4.5 s.Time isinseconds.Shownat2 frames/s. B, Isosurface reconstructionof the spreading permeabilization(see Fig. 6).A starfish oocyte injectedwith Alexa 488 500-kDdextranwasimagedduring maturationinthree dimensionsover time.Isosurface reconstructionof the complete dataset.IntactNE, lightgreen; permeabilizedareas, dark, transparentgreen.Stackswere acquiredevery 4.5 s.Time isinseconds.Shownat2 frames/s.
