Video 4.

Inhibitionof Cdc42 preventsformationof a persistentpseudopodorientedtowarda micropipette source of fMLP.Thiscell, expressing PH-Akt-GFP andCdc42N17, wasexposedto fMLP suppliedby a micropipette locateddirectly below the cell.Itsinitial response isto form two small pseudopodsina more or lesscorrectorientation, thento fuse them, andthento form a pseudopodinanirrelevantorientation.Imageswere takenevery 8 sandare displayedat2 frames/s.

Rac and Cdc42 play distinct roles in regulating PI(3,4,5)P3 and polarity during neutrophil chemotaxis

Supriya Srinivasan , Fei Wang , Suzana Glavas , Alexander Ott , Fred Hofmann , Klaus Aktories , Daniel Kalman , and Henry R. Bourne

DOI: 10.1083/jcb.200208179