Video 2.

Ca²⁺ changesduring directedß2-mediatedphagocytosis.The video clip showsthe changesincytosolic free Ca²⁺ ina humanneutrophil during phagocytosis, pseudocoloredfrom blue (low Ca²⁺, 100 nM) to green(high Ca²⁺, 700 nM; (middle).(Left) Historic Ca²⁺ concentrationinthe cell, plottedgraphically.(Right) Phase contrastimage of the cell.The micropipette isseeninthe early partof the sequence presenting the C3bi-opsonizedzymosanparticle to the neutrophil which bindsthe particle, formsa phagocytic cup andundergoesphagocytosis.Inthisexample, the Ca²⁺ signal isclearly double peakedandcorrelateswith the accelerationphase of the phagocytosis.

Cytosolic free Ca2+ changes and calpain activation are required for β integrin–accelerated phagocytosis by human neutrophils

Sharon Dewitt , and Maurice B. Hallett

DOI: 10.1083/jcb.200206089