Correspondsto Fig. 2 A.A time-lapse video sequence of anEGFP-topoIIα-expressing GT2-LPk cell imagedfrom late prometaphase through telophase. Chromosomesalignatthe metaphase plate with EGFP-topoIIα concentratedatkinetochoresandalong the axesof the chromosome arms. Aschromosomesseparate atanaphase, the kinetochore concentrationdisappears. Inlate anaphase, the levelsof EGFP-topoIIα rise inthe cytoplasm, with some concentratedinto foci thatare motile and, attelophase, appear to fuse with the reforming nuclei. A phase contrastimage of the two daughter cellsisshownatthe endof the sequence. The fluorescence imagesinthissequence were individually scaledinorder to optimize visualizationof the EGFP-topoIIα cytoplasmic foci that appear inanaphase and telophase.
