Video 1.

VSVG-GFP inthe Golgi complex (FRAP). COS7 cellswere transfectedwith VSVG-GFP, placedonglass-bottom microwell disheswith coordinatedgrids, subjectedto small-pulse protocol, andexaminedby laser scanning confocal microscope.4 minafter the shiftfrom 15°C to 40°C, the Golgi spotscontaining VSVG-GFP inthe central Golgi area were maskedby a high-ER background.To remove thisbackground, the whole cell, exceptthe Golgi area, wasrepeatedly bleached.Thismade the fluorescentspotsinthe Golgi zone more evident.These spotshoveredwithinthe Golgi area withoutexhibiting long-range movements.Next, at6-7 min, half of the Golgi area wasbleachedto examine whether fluorescence wouldrecover inthe bleachedspots.No recovery wasobserved.10 minafter the shiftfrom 15°C to 40°C, the GFP-containing spotsbecame more mobile andbeganto leave the Golgi area.

Small cargo proteins and large aggregates can traverse the Golgi by a common mechanism without leaving the lumen of cisternae

Alexander A. Mironov , Galina V. Beznoussenko , Paolo Nicoziani , Oliviano Martella , Alvar Trucco , Hee-Seok Kweon , Daniele Di Giandomenico , Roman S. Polishchuk , Aurora Fusella , Pietro Lupetti , Eric G. Berger , Willie J.C. Geerts , Abraham J. Koster , Koert N.J. Burger , and Alberto Luini

DOI: 10.1083/jcb.200108073