DC movement in RvE1-treated CD11c-YFP mice in the steady state. A representative video showing DC movement in the steady state skin of RvE1-treated CD11c-YFP mice by two-photon microscopy. The bright green signals indicate CD11c-YFP–positive cells (DCs), and blue signals indicate collagen fibers in the skin. RvE1 (200 ng/mouse) was administered intravenously at 60 min. The timing of RvE1 administration is indicated at the top left. DC motility gradually decreased 30 min after RvE1 treatment (time lapse over 240 min; 240 frames, 30 frames/s). Bar, 200 µm. Data are representative of three independent experiments with reproducible results.
