LLSM of tent pole ruffles. RAW 264.7 macrophages stably expressing GFP-LifeAct stimulated with LPS. Video of Fig. 2 A visualized in 3D (tilted) to visualize top and side of the cell. GFP-LifeAct fluorescence is visualized using the Blue-Green LUT using Amira visualization software. Frames were collected every 3.10 s and are displayed as 20 fps. Bars, 10 µm. Time stamp, min:s.
