Local Rho activity peaks precede maximal actin and Myosin IIa accumulation. (Top) Time-lapse TIRF video of the Rho activity sensor mCherry-Rhotekin-GBD (left) and EGFP-actin (right) in a representative U2OS cell. (Middle and bottom) Time-lapse TIRF video of the Rho activity sensor mCherry-Rhotekin-GBD (left) and Myosin IIa (EGFP-NMHCIIa, right) in a representative U2OS cell. Bottom panels show untreated, resting cells; images in the upper and middle panels were collected 45–90 min after treatment with 30 µM nocodazole using a frame rate of 20/min. Bars, 10 µm.
