Video 2.

FiloQuant analysis of a movie depicting DCIS.COM cell invasion through fibrillar collagen (circular invasion assay). The original video (input), in addition to the multiple output files generated by FiloQuant, is displayed. Output files include filopodia detection (magenta), tracking image (filopodia dynamics), skeleton (mask used for filopodia detection), edge detection (defining the filopodia-free cell edge) and contour detection (to measure edge length).


FiloQuant reveals increased filopodia density during breast cancer progression

Guillaume Jacquemet, Ilkka Paatero, Alexandre F. Carisey, Artur Padzik, Jordan S. Orange, Hellyeh Hamidi, and Johanna Ivaska

DOI: 10.1083/jcb.201704045
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