Video 2

Cytoplasmic mixing between three cells expressing HAP2 and RFPcyto. Three cells in the middle (boxed) merge their cytoplasms around time 3:15. We then confirmed that the syncytium contained three nuclei using multifocal sectioning by spinning disc confocal microscopy (Fig. S1). Another cell undergoes karyokinesis around time 3:45 (arrow). See still images in Fig. 1 D.


Arabidopsis HAP2/GCS1 is a gamete fusion protein homologous to somatic and viral fusogens

Clari Valansi, David Moi, Evgenia Leikina, Elena Matveev, Martín Graña, Leonid V. Chernomordik, Héctor Romero, Pablo S. Aguilar, and Benjamin Podbilewicz

DOI: 10.1083/jcb.201610093
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