PtK1 cells expressing Hec1-GFP treated with NOC. (left) Live-cell microscopy of a PtK1 mitotic cell treated with NOC. The cell expressing Hec1 labeled with GFP (green) was injected with α-tubulin–X-rhodamine (magenta). Time 0 indicates the frame before addition of 9 µM NOC. Images were acquired at 20-s intervals. The video is displayed at 10 fps. Bar, 5 µm. The video corresponds to Fig. 3 G. (right) Separate green channel showing PtK1 KTs (Hec1-GFP).
