Clb2 dynamics in adapting cells. CLB2-GFP TUB2-Cherry GAL1-MAD2 (3X) (yAC1732) cells were grown in synthetic medium containing raffinose and then shifted to galactose .We show Clb2-GFP accumulation signal in the nucleus after smoothing. Time-lapse movies were acquired every 10 min using a DeltaVision Elite imaging system (Applied Precision) based on an inverted microscope (IX71; Olympus).
