3D reconstruction of the wild-type staining pattern of Hts-RC in an egg chamber at stage 8; related to Fig. 4. 3D reconstruction of ring canals in a wild-type Drosophila egg chamber at stage 8, which were stained for Hts-RC (white). Not all ring canals are visible because the image stack is restricted to the central region of the egg chamber. The reconstruction is derived from still images obtained using a confocal microscope (LSM 510 Meta).