Imaging of full-length polycystin-2. LLC-PK1 cells stably synthesizing an ECFP fusion protein with N-acetylgalactosaminyltransferase-2 (shown in red for better visibility) were transiently transfected with an expression plasmid coding for an EGFP fusion protein with full-length polycystin-2. 3 d later the cells were incubated for 5 h at 15°C before being incubated at 37°C. Pictures were taken every 2 min over a period of 60 min, ∼5 min after shifting the cells to 37°C. No overlapping signal can be seen between full-length polycystin-2 and N-acetylgalactosaminyltransferase-2.
