Monitoring of cell fate upon serum starvation. RPE1 cells were plated on large, 500-µm-wide, fibronectin-coated discoidal micropatterns and monitored in 10× time-lapse phase-contrast microscopy to measure cell division time with respect to the time of starvation and observe daughter cell fates. Time interval between two frames is 10 min. Colored dots are used as visual marks to follow individual cell fates. The blue cell’s daughters were starved in late G1 and divided again. The red cell’s daughters were starved in early G1 and didn’t divide again. The green cell’s daughters adopted asymmetric behaviors: one divided again and not the other.
